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General information |
Course unit name: Fluorescence Microscopy Techniques
Course unit code: 569907
Academic year: 2021-2022
Coordinator: Carles Rentero Alfonso
Department: Faculty of Medicine
Credits: 3
Single program: S
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Prior considerations |
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http://www.olympusmicro.com/primer/techniques/fluorescence/fluorhome.html |
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Estimated learning time |
Total number of hours 75 |
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Face-to-face and/or online activities |
33 |
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- Lecture |
Face-to-face and online |
5 |
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- Lecture with practical component |
Face-to-face and online |
6 |
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- Group tutorial |
Face-to-face and online |
5 |
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- IT-based class |
Face-to-face and online |
2 |
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- Laboratory session |
Face-to-face |
15 |
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Supervised project |
15 |
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Independent learning |
27 |
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Competences to be gained during study |
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— Basic knowledge of the physical and biological bases of fluorescence microscopy techniques.
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Learning objectives |
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Referring to knowledge — Describe fluorescence microscopy’s physical and biological background.
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Teaching blocks |
1.
Characteristics of visual information in biomedicine; Basic and optical principles; 2D and 3D resolution; Light sources; Fluorescence; Fluorochromes; Fluorescence microscopy; Photobleaching: applications
2.
Bioimaging; Fluorescent labelling in proliferation and apoptosis; Monitoring gene function by fluorescent genetic markers; Multiple fluorescent labelling; New generation of fluorescent molecules; Loss and gain of gene function studies with fluorescence microscopy; Applications in biomedical research; Brainbow: genetic design for non invasive cell tracing
3.
Microscopy of living cells; How to maintain living cells under a microscope; Molecular labelling with fluorescence; Transmitted light contrast techniques; Capture of images in time intervals (time lapse microscopy)
4.
Fluorescence photobleaching; Study of molecular dynamics; FRAP (Fluorescence recovery after photobleaching) and iFRAP (inverse FRAP); FLIP (Fluorescence loss in photobleaching); Fluorescent labelling; How to perform an experiment of photobleaching: microscope settings, parameters to consider; Image analysis and kinetic parameters
5. Resonance energy transfer between molecules in fluorescent microscopy (FRET, Fluorescence resonance energy transfer); Study of molecular interaction; Concepts; Methodology: acceptor photobleaching method; Labelling molecular FRET pairs; How to perform an experiment with FRET microscopy: settings, microscope parameters to take into account; Image analysis, representation and interpretation of results
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Teaching methods and general organization |
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Lectures |
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Official assessment of learning outcomes |
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Students are assessed on their acquisition of theoretical and practical knowledge. Attendance, participation in proposed activities and acquired abilities are also evaluated.
Examination-based assessment Students are assessed on their acquisition of theoretical and practical knowledge. Attendance, participation in proposed activities and acquired abilities are also evaluated. |
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Reading and study resources |
Consulteu la disponibilitat a CERCABIB
Book
Current Protocols in Cell Biology. John Wiley and Sons. Somerset, NJ. ISSN 1934-3639. 2012. http://www.currentprotocols.com/WileyCDA/Section/id-810292.html
Live Cell Imaging: A Laboratory Manual Robert D. Goldman, David L. Spector, Jason R. Swedlow (Editor) Cold Spring Harbor Laboratory Press; 2 edition (December 30, 2009) Live Cell Imaging: Methods and Protocols (Methods in Molecular Biology) [Hardcover] Dmitri B. Papkovsky (Editor) Humana Press; 1st Edition. edition (December 17, 2009)