Teaching plan for the course unit (Short version)
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General information

 

Course unit name: Laboratory Practice in Human Biology II

Course unit code: 568582

Academic year: 2021-2022

Coordinator: Barbara Arias Samperiz

Department: Department of Evolutionary Biology, Ecology and Environmental Sciences

Credits: 2,5

Single program: S

Estimated learning time

Total number of hours 62.5

 

Face-to-face and/or online activities

25

	-  Lecture	Face-to-face		10
	-  Laboratory session	Face-to-face		15

Supervised project

16

Independent learning

21.5

Learning objectives

Referring to knowledge — Design a laboratory protocol. — Extract biological material from human samples. — Use PCR to amplify DNA sequences. — Use electrophoresis to distinguish between DNA samples. — Calculate genetic variability parameters.

 

 

Teaching blocks

 

Session 1 (2 hours)

*  

• Introduction to the course (organisation of the sessions and course objectives)
• Organising student work groups
• Assigning tasks to group members

Sessions 2, 3 and 4 (2 hours each)

*  

• Reporting back to work groups and meetings with specialists
• Introduction to the practicals format
• Assigning tasks related to the execution of the protocol
• Tutorials about the tasks being completed by different work groups
• Presentation of the preliminary version of the protocol

Note that the organisation of the sessions will depend on the number of students enrolled.

Session 5 (2 hours)

*  

• Presentation of the final version of the protocol

Note that the organisation of the sessions will depend on the number of students enrolled.

Laboratory sessions

*  Session 1

• Obtaining biological samples
• DNA extraction using an extraction kit
• Measuring DNA quantity and quality
  
  • DNA marker systems

Session 2

• Genotyping single nucleotide polymorphisms
  
  • PCR
  • Agarose gel analysis for verification of PCR products
  • Restriction enzyme digestion

Session 3

• Genotyping single nucleotide polymorphisms
  
  • Preparation of agarose gels
  • Interpreting genotypes
• Analysis of results
  
  • Allele frequency
  • Genotype frequency
  • The Hardy–Weinberg principle

*Note that the organisation of the sessions will depend on the number of students enrolled.

COVID-19

*  Note that the total completion of this content may be subject to official COVID-19 restrictions, but that the laboratory sessions will need to be completed in face-to-face mode.