Teaching plan for the course unit (Short version)
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General information

 

Course unit name: Molecular Techniques Applied to Anthropology

Course unit code: 574173

Academic year: 2021-2022

Coordinator: Araceli Rosa de la Cruz

Department: Department of Evolutionary Biology, Ecology and Environmental Sciences

Credits: 2,5

Single program: S

Estimated learning time

Total number of hours 62.5

 

Face-to-face and/or online activities

25

	-  Lecture	Face-to-face		8
	-  IT-based class	Face-to-face		3
	-  Laboratory session	Face-to-face		5
	-  Field trip	Face-to-face		3
	-  Seminar	Face-to-face		6

Supervised project

20

Independent learning

17.5

Learning objectives

Referring to knowledge — Explain the principles underlying different techniques used in the genomic sequencing (genotyping) of biological samples. — Identify the techniques that are most frequently used in the anthropogenetics laboratory. — Describe and discuss the theoretical and practical advantages of the various molecular techniques used in the ambit of anthropology.

 

 

Teaching blocks

 

1. Small-scale SNP genotyping

*  Tools and platforms such as the SNPlex Genotyping System and real time PCR (quantitative PCR, qPCR)

2. Large-scale SNP genotyping

*  SNP matrix technologies such as Sequenom and Genome-Wide

3. DNA sequencing techniques

*  Sanger sequencing, pyrosequencing and next-generation sequencing. Applications in anthropological studies.

4. Epigenetics analysis

5. Laboratory session

*  A five-hour molecular lab session to work on SNP genotyping using TaqMan PCR

6. Computer session

*  A three-hour computer session to process the data generated in the laboratory session and prepare it for statistical treatment.

(Note that this class is taught in English.)

7. Seminars

*  Session 1: A talk by a specialist and follow-up discussion on the use of next-generation sequencing or mass genotyping methodologies in recent studies

Session 2: Preparation of student seminars

8. Guided visit

*  Students visit the Universitat Pompeu Fabra’s Genomics Core Facility in the Barcelona Biomedical Research Park (PRBB)

 

 

Reading and study resources

Check availability in Cercabib

Book

Molecular Methods for Evolutionary Genetics

 

ORGOGOZO, V; ROCKMAN, M. ed. Molecular Methods for Evolutionary Genetics. Humana Press. 2011. DOI https://doi.org/10.1007/978-1-61779-228-1